IIT Bombay Using mango leaves to synthesise fluorescent graphene quantum dots

The quantum dots can be used for bioimaging intracellular temperature

Unlike the now employed dyes, quantum dots have excellent equilibrium synthesized from mango leaves are biocompatible and show no cellular toxicity.

Green path

Mango leaves cut into tiny pieces and froze them using liquid nitrogen to synthesize quantum dots. The frozen leaves dipped in alcohol and were crushed into powder.

Using mice fibroblast cells, a team directed by Rohit Srivastava from the Department of Biosciences and Bioengineering at IIT Bombay appraised the potential of quantum dots for bioimaging and temperature-sensing applications. In mice cell in vitro studies, the quantum dots that are graphene could get into the cells easily without destroying the integrity, viability, and multiplication of the cells. The quantum dots get into the cytoplasm of the cell.

The quantum dots, 2-8 nanometre in size, were found to emit red luminescence when excited by UV light. When the excitation wavelength was 300-500 nanometre, the emission in the quantum dots was at 680 nanometre. The quantum dots demonstrated excitation- independent emission,” says Mukeshchand Thakur from the Department of Biosciences and Bioengineering at IIT Bombay, one of the authors of the paper.

The quantum dots have larger and smaller fluorescent units. When the excitation is at a lower wavelength, energy is transferred by the smaller units to the bigger units, and there is red emission. And when the excitation is at a higher wavelength, the red emission comes straight from the larger units, consequently remaining excitation-independent.

Nanothermometer

The graphene quantum dots can be used for cell cytoplasm labeling applications Since the quantum dots get into the cytoplasm of the cell,” says the initial author of the paper, IIT Bombay and Mukesh Kumar Kumawat of Biosciences and Bioengineering.

Extreme florescence was shown by the quantum dots found inside the cells The strength of fluorescence will decrease as the temperature rises to 45 degrees C.

Because of this, the researchers found up to 95% decrease in fluorescence intensity when 20 degrees C. increased the temperature “So quantum dots may be used for discovering temperature variation ” says Thakur.

The graphene quantum dots can be utilized as a nanothermometer. Besides quantifying intracellular temperature increase, they may be used for quantifying temperature increase in cancer cells and when there is inflammation,” says Prof. Srivastava. “we're seeing interest by businesses making imaging probes. There is also interest to work with it as a temperature probe.”

They can be used for making organic light Since the quantum dots emit light that was red -emitting diodes too, ” says Kumawat.

Mango leaves quantum dots bioimaging